Department of

Plant Biophysics & Biochemistry

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  <In databases, "Küpper" is usually written as "Kupper", rarely as "Kuepper">

Full-length original research articles; *=corresponding author

<Links are either directly to a pdf reprint if the article is open access, otherwise they are to the article on the journal website>

(71) Ashraf N, Vitova M, Cloetens P, Mijovilovich A, Bokhari SNH, Küpper H* (2021) Effect of nanomolar concentrations of lanthanum on Desmodesmus quadricauda cultivated under environmentally relevant conditions. Aquatic Toxicology (online ahead of print,

(70) Morina F*, Mijovilovich A, Koloniuk I, Pěnčík A, Grúz J, Novák O, Küpper H* (2021) Interactions between zinc and Phomopsis longicolla infection in the roots of Glycine max. Journal of Experimental Botany 72, 3320–3336,

(69) Takahama U*, Hirota S, Morina F (2020) Slower liberation and digestion of amylose in high-amylose rice cooked with adzuki bean: contribution of procyanidins. European Food Research and Technology,

(68) Sinha RK*, Verma SS, Rastogi A* (2020) Role of Pathogen-Related Protein 10 (PR 10) under abiotic and biotic stresses in Plants. Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2020.09359

(67) Duran P, Barra PJ, de la Luz Mora M, Morina F, Viscardi Sh, Meriño-Gergichevich C* (2020) First report of fungal complex causing grey necrosis of hazelnut in Chile. New Disease Reports 42, 7.

(66) Castro-Rodríguez R, Abreu I, Reguera M, Novoa-Aponte L, Mijovilovich A, Escudero V, Jiménez-Pastor FJ, Abadía J, Wen J, Mysore KS, Álvarez-Fernández A, Küpper H, Imperial J, González-Guerrero M* (2020) Medicago truncatula Yellow Stripe1-Like3 gene is involved in vascular delivery of transition metals to root nodules. Journal of Experimental Botany 71, 7257-69 eraa390,

(65) Morina F, Küpper H* (2020) Direct inhibition of photosynthesis by Cd dominates over inhibition caused by micronutrient deficiency in the Cd/Zn hyperaccumulator Arabidopsis halleri. Plant Physiology and Biochemistry 155_252-261, DOI:

(64) Mijovilovich A, Morina F, Bokhari SNH, Wolff T, Küpper H* (2020) Analysis of trace metal distribution in plants with lab-based microscopic X-ray fluorescence imaging, Plant Methods, DOI: 10.1186/s13007-020-00621-5

(63)  Morina F, Mishra A, Mijovilovich A, Matoušková Š, Brückner D, Špak J, Küpper H* (2020)  Interaction between Zn deficiency, toxicity and Turnip yellow mosaic virus infection in Noccaea ochroleucum. Frontiers in Plant Science, section Plant Physiology, DOI:

(62) Nostadt R, Hilbert M, Nizam S, Rovenich H, Wawra S, Martin J, Küpper H, Mijovilovich A, Ursinus A, Langen G, Hartmann MD, Lupas AN, Zuccaro A* (2020) A secreted fungal histidine‐ and alanine‐rich protein regulates metal ion homeostasis and oxidative stress. New Phytologist 227(4), 1174-1188, DOI:

(61) Morina F, Hirota S, Takahama U* (2020) Contribution of amylose-procyanidin complexes to slower starch digestion of red-colored rice prepared by cooking with adzuki bean. International Journal of Food Sciences and Nutrition 71, 715-725, DOI:

(60) Meriño-Gergichevich C*, Morina F, Jorquera-Fontena E, Seguel A (2020) Differential tolerance and phenolics leaf profile in response to boron supply in two highbush blueberry genotypes. Journal of Soil Science and Plant Nutrition 20, 610–620 , DOI:

(59) Andresen E, Lyubenova L, Hubáček T, Bokhari SNH, Matoušková Š, Mijovilovich A, Rohovec J, Küpper H* (2020) Chronic exposure of soybean plants to nanomolar cadmium reveals specific additional high-affinity targets of Cd toxicity. Journal of Experimental Botany 71, 1628-1644, DOI:

(58) Küpper H*, Bokhari SNH, Jaime-Pérez N, Lyubenova L, Ashraf N, Andresen E (2019) Ultratrace metal speciation analysis by coupling of sector-field ICP-MS to high-resolution size exclusion and reversed-phase liquid chromatography. Analytical Chemistry 91, 1710961-10969, DOI: 10.1021/acs.analchem.9b00222)

(57) Jaime-Pérez N, Kaftan D, Bína D, Bokhari SNH, Shreedhar S, Küpper H* (2019) Mechanisms of sublethal copper toxicity damage to the photosynthetic apparatus of Rhodospirillum rubrum. Biochimica et Biophysica Acta (BBA) - Bioenergetics 1860, 640-650, DOI:

(56) Mijovilovich A, Mishra A, Brückner D, Spiers K, Andresen E, Garrevoet J, Falkenberg G, Küpper H* (2019) MicroX-ray absorption near edge structure tomography reveals cell-specific changes of Zn ligands in leaves of turnip yellow mosaic virus infected plants. Spectrochimica Acta Part B: Atomic Spectroscopy 157, 53-62, DOI:

(55) Takahama U*, Hirota S, Morina F (2019) Procyanidins in rice cooked with adzuki bean and their contribution to the reduction of nitrite to nitric oxide (•NO) in artificial gastric juice. International Journal of Food Sciences and Nutrition,

(54) Küpper H*, Benedikty Z, Morina F, Andresen E, Mishra A, Trtílek M (2019) Analysis of OJIP chlorophyll fluorescence kinetics and QA re-oxidation kinetics by direct fast imaging. Plant Physiology 179, 369-381, DOI:

(53) Sedlarević A, Morina F*, Toševski I, Tosti T, Jović J, Krstić O, Veljović‐Jovanović S (2019) Resource allocation in response to herbivory and gall formation in Linaria vulgaris. Plant Physiology and Biochemistry 135, 224-232,

(52) Mishra AK, Kumar A, Mishra D, Nath VS, Jakše J, Kocábek T, Killi UK, Morina F, Matoušek J* (2018) Genome-Wide Transcriptomic Analysis Reveals Insights into the Response to Citrus bark cracking viroid (CBCVd) in Hop (Humulus lupulus L.). Viruses 10, 570; doi:10.3390/v10100570.

(51) Mishra S, Mishra A, Küpper H* (2017) Protein Biochemistry and Expression Regulation of Cadmium/Zinc Pumping ATPases in the Hyperaccumulator Plants Arabidopsis halleri and Noccaea caerulescens. Frontiers in Plant Scince,

(50) Morina F*, Vidović M, Srećković T, Radović V, Veljović‑Jovanović S (2017) Biomonitoring of urban pollution using silicon-accumulating species, Phyllostachys aureosulcata ‘Aureocaulis’. Bull Environ Contam Toxicol 99, 706–712.

(49) Eroglu S, Giehl RFH, Meier B, Takahashi M, Terada Y, Ignatiev K, Andresen E, Küpper H, Peiter E, von Wirén N* (2017) Metal Tolerance Protein 8 mediates manganese homeostasis and iron re-allocation during seed development and germination. Plant Physiology 174, pp. 1633–1647.

(48) Mishra S, Alfeld M, Sobotka R, Andresen E, Falkenberg G, Küpper H* (2016) Analysis of sublethal arsenic toxicity to Ceratophyllum demersum: Subcellular distribution of arsenic and inhibition of chlorophyll biosynthesis. Journal of Experimental Botany 67, 4639-4646.

(47) Thomas G, Andresen E, Mattusch J, Hubáček T, Küpper H* (2016) Deficiency and toxicity of nanomolar copper in low irradiance – a physiological and metalloproteomic study in the aquatic plant Ceratophyllum demersum. Aquatic Toxicology 177, 226–236

(46) Andresen E, Kappel S, Stärk HJ, Riegger U, Borovec J, Mattusch J, Heinz A, Schmelzer CEH, Matoušková Š, Dickinson B, Küpper H* (2016) Cadmium toxicity investigated at the physiological and biophysical levels under environmentally relevant conditions using the aquatic model plant Ceratophyllum demersum L. New Phytologist 210, 1244-1258.

(45) Mishra S, Stärk H-J, Küpper H* (2014) A different sequence of events than previously reported leads to arsenic-induced damage in Ceratophyllum demersum L. Metallomics 6, 444-454

(44) Mishra S*, Wellenreuther G, Mattusch J, Stärk H-J, Küpper H (2013) Speciation and distribution of arsenic in the non-hyperaccumulator macrophyte Ceratophyllum demersum L. Plant Physiology 163, 1396-1408

(43) Andresen E, Opitz J, Thomas G, Stärk H-J, Dienemann H, Jenemann K, Dickinson BC, Chang CJ, Küpper H* (2013) Effects of Cd & Ni toxicity to Ceratophyllum demersum under environmentally relevant conditions in soft & hard water including a German lake.  Aquatic Toxicology 142-143, 387-402

(42) Ferimazova N, Felcmanová K, Šetlíková E, Küpper H, Maldener I, Hauska G, Šedivá B, Prášil O* (2013) Regulation of photosynthesis during heterocyst differentiation in Anabaena sp. strain PCC 7120 investigated in vivo at single-cell level by chlorophyll fluorescence kinetic microscopy. Photosynthesis Research 116:79–91

(41) Andresen E, Mattusch J, Wellenreuther G, Thomas G, Abad UA, Küpper H* (2013) Different strategies of cadmium detoxification in the submerged macrophyte Ceratophyllum demersum L.. Metallomics, 5, 1377-1386

(40) Peng H, Kroneck PMH, Küpper H* (2013) Toxicity and deficiency of copper in Elsholtzia splendens affect photosynthesis biophysics, pigments and metal accumulation. Environmental Science and Technology 47, 6120-6128.

(39) Thomas G, Stärk H-J, Wellenreuther G, Dickinson BC, Küpper H* (2013) Effects of nanomolar copper on water plants - comparison of biochemical and biophysical mechanisms of deficiency and sublethal toxicity under environmentally relevant conditions. Aquatic toxicology 140-141, 27-36

(38) Peng H, Wang-Müller Q, Witt T, Malaisse F, Küpper H*. (2012) Differences in copper accumulation and copper stress between eight populations of Haumaniastrum katangense. Environmental and Experimental Botany 79, 58-65.

(37) Leitenmaier B, Witt A, Witzke A, Stemke A, Meyer-Klaucke W, Kroneck PMH, Küpper H* (2011) Biochemical and biophysical characterisation yields insights into the mechanism of TcHMA4, a Cd/Zn transporting ATPase purified from the hyperaccumulator plant Thlaspi caerulescens. Biochimica et Biophysica Acta (section Biomembranes) 1808, 2591-2599.

(36) Lohscheider JN, Strittmatter M, Küpper H, Adamska I*. (2011) Vertical Distribution of Epibenthic Freshwater Cyanobacterial Synechococcus spp. Strains Depends on Their Ability for Photoprotection. PLoS ONE 6(5): e20134. doi:10.1371/journal.pone.0020134.

(35) Leitenmaier B, Küpper H* (2011) Cadmium uptake and sequestration kinetics in individual leaf cell protoplasts of the Cd/Zn hyperaccumulator Thlaspi caerulescens. Plant Cell and Environment 34, 208-219

(34) Trampczynska A, Küpper H, Meyer-Klaucke W, Schmidt H, Clemens S* (2010) Nicotianamine forms complexes with Zn(II) in vivo. Metallomics 2, 57-66

(33) Andresen E, Lohscheider J, Šetlikova E, Adamska I, Šimek M, Küpper H* (2010) Acclimation of Trichodesmium erythraeum ISM101 to high and low irradiance analysed on the physiological, biophysical and biochemical level. New Phytologist 185, 173-188

(32) Küpper H*, Kochian LV (2010) Transcriptional regulation of metal transport genes and mineral nutrition during acclimation to Cadmium and Zinc in the Cd/Zn hyperaccumulator, Thlaspi caerulescens (Ganges population). New Phytologist 185, 114-129

(31) Mijovilovich A, Leitenmaier B, Meyer-Klaucke W, Kroneck PMH, Götz B, Küpper H* (2009) Complexation and toxicity of copper in higher plants (II): Different mechanisms for Cu vs. Cd detoxification in the Cu-sensitive Cd/Zn hyperaccumulator Thlaspi caerulescens (Ganges ecotype). Plant Physiology 151, 715-731

(30) Küpper H*, Mijovilovich A, Götz B, Küpper FC, Meyer-Klaucke W (2009) Complexation and toxicity of copper in higher plants (I): Characterisation of copper accumulation, speciation and toxicity in Crassula helmsii as a new copper hyperaccumulator. Plant Physiology 151, 702-714

(29) Rocchetta I, Küpper H* (2009) Chromium and Copper induced inhibition of photosynthesis in Euglena gracilis analysed on the single-cell level by fluorescence kinetic microscopy. New Phytologist 182, 405-420.

(28) Küpper H*, Andresen E, Wiegert S, Šimek M, Leitenmaier B, Šetlík I (2009) Reversible coupling of individual phycobiliprotein isoforms during state transitions in the cyanobacterium Trichodesmium analysed by single-cell fluorescence kinetic measurements. Biochimica et Biophysica Acta (section Bioenergetics) 1787, 155-167.

(27) Küpper H*, Šetlík I, Seibert S, Prášil O, Šetlikova E, Strittmatter M, Levitan O, Lohscheider J, Adamska I, Berman-Frank I (2008) Iron limitation in the marine cyanobacterium Trichodesmium reveals new insights into regulation of photosynthesis and nitrogen fixation. New Phytologist 179, 784-798.

(26) Parameswaran A#, Leitenmaier B#, Yang M, Welte W, Kroneck PMH, Lutz G, Papoyan A, Kochian LV, Küpper H* (2007) A native Zn/Cd transporting P1B type ATPase protein from natural overexpression in a Zn/Cd hyperaccumulator plant. Biochemical and Biophysical Research Communications 363, 51-56.
# the first two authors contributed equally to this study

(25) Küpper H*, Seibert S, Aravind P (2007) A fast, sensitive and inexpensive alternative to analytical pigment HPLC: quantification of chlorophylls and carotenoids in crude extracts by fitting with Gauss-Peak-Spectra. Analytical Chemistry 79, 7611-7627.

(24) Küpper H*, Aravind P, Leitenmaier B, Trtílek M, Šetlík I (2007) Cadmium-induced inhibition of photosynthesis and long-term acclimation to Cd-stress in the Cd hyperaccumulator Thlaspi caerulescens. New Phytologist 175, 655-674.

(23) Küpper H, Seib LO, Sivaguru M, Hoekenga O, Kochian LV* (2007) A method for cellular localisation of gene expression via quantitative in situ hybridisation in plants. Plant Journal 50, 159-187.

(22) Lu S, Van Eck J, Zhou X, Lopez AB, O'Halloran DM, Cosman KM, Conlin BJ, Paolillo DJ, Garvin DF, Vrebalov J, Kochian LV, Küpper H, Earle ED, Cao J, Li L* (2006) The cauliflower Or encodes a DnaJ cysteine-rich domain-containing protein that mediates high-levels of carotene accumulation. Plant Cell 18, 3594-3605.

(21) Gachon CMM*, Küpper H, Küpper FC, Šetlík I (2006) Chlorophyll fluorescence kinetic microscopy of Pylaiella littoralis (Phaeophyceae) infected by Chytridium polysiphoniae (Chytridiomycota).  European Journal of Phycology 41, 395-403.

(20) Šetlíková E, Šetlík I*, Küpper H, Kasalický V, Prášil O (2005) The photosynthesis of individual algal cells during the cell cycle of Scenedesmus quadricauda studied by chlorophyll fluorescence kinetic microscopy. Photosynthesis Research 84, 113-120.

(19) Küpper H*, Šetlík I, Hlásek M (2004) A versatile chamber for simultaneous measurements of oxygen exchange and fluorescence in filamentous and thallous algae as well as higher plants. Photosynthetica 42, 579-583.

(18) Küpper H*, Ferimazova N, Šetlík I, Berman-Frank I (2004) Traffic lights in Trichodesmium: regulation of photosynthesis for nitrogen fixation studied by chlorophyll fluorescence kinetic microscopy. Plant Physiology 135(4), 2120-2133.

(17) Küpper H*, Mijovilovich A, Meyer-Klaucke W, Kroneck PMH (2004) Tissue- and age-dependent differences in the complexation of cadmium and zinc in the Cd/Zn hyperaccumulator Thlaspi caerulescens (Ganges ecotype) revealed by X-ray absorption spectroscopy. Plant Physiology 134(2), 748-757.

(16) Carr HP, Lombi E, Küpper H, McGrath SP, Wong MH* (2003) Accumulation and distribution of aluminium and other elements in tea (Camellia sinensis) leaves. Agronomie 23(8), 705-710.

(15) Küpper H*, Šetlík I, Šetliková E, Ferimazova N, Spiller M, Küpper FC (2003) Copper-induced inhibition of photosynthesis: limiting steps of in vivo copper chlorophyll formation in Scenedesmus quadricauda. Functional Plant Biology 30(12), 1187-1196.

(14) Ferimazova N, Küpper H*, Nedbal L, Trtílek M (2002) New insights into photosynthetic oscillations revealed by two-dimensional microscopic measurements of chlorophyll fluorescence kinetics in intact leaves and isolated protoplasts. Photochemistry and Photobiology 76(5), 501-508.

(13) Küpper H*, Dìdic R, Svoboda A, Hála J, Kroneck PMH (2002) Kinetics and efficiency of excitation energy transfer from chlorophylls, their heavy metal substituted derivatives, and pheophytins to singlet oxygen. Biochimica et Biophysica Acta 1572 (1), 107-113.

(12) Küpper H*, Šetlík I, Spiller M, Küpper FC, Prášil O (2002) Heavy metal-induced inhibition of photosynthesis: targets of in vivo heavy metal chlorophyll formation. Journal of Phycology 38(3), 429-441.

(11) Küpper H, Lombi E, Zhao FJ, Wieshammer G, McGrath SP* (2001) Cellular compartmentation of nickel in the hyperaccumulators Alyssum lesbiacum, Alyssum bertolonii and Thlaspi goesingense. Journal of Experimental Botany 52(365), 2291-2300.

(10) Berman-Frank I*, Lundgren P, Chen Yi-B, Küpper H, Kolber Z, Bergman B, Falkowski P (2001) Segregation of nitrogen fixation and oxygenic photosynthesis in the marine cyanobacterium Trichodesmium. Science 294, 1534-1537.

(9) Küpper H, Lombi E, Zhao FJ, McGrath SP* (2000) Cellular compartmentation of cadmium and zinc in relation to other elements in the hyperaccumulator Arabidopsis halleri. Planta 212, 75-84

(8) Küpper H*, Spiller M, Küpper F (2000) Photometric method for the quantification of chlorophylls and their derivatives in complex mixtures: fitting with gauss-peak-spectra. Analytical Biochemistry 286, 247-256.

(7) Küpper H*, Šetlík I, Trtílek M, Nedbal L (2000) A microscope for two-dimensional measurements of in vivo chlorophyll fluorescence kinetics using pulsed measuring light, continuous actinic light and saturating flashes. Photosynthetica 38(4), 553-570.

(6) Küpper H, Zhao F, McGrath SP* (1999) Cellular compartmentation of zinc in leaves of the hyperaccumulator Thlaspi caerulescens. Plant Physiology 119, 305-311.

(5) Müller DG*, Küpper FC, Küpper H (1999) Infection experiments reveal broad host ranges of Eurychasma dicksonii (Oomycota) and Chytridium polysiphoniae (Chytridiomycota), two eukaryotic parasites in marine brown algae (Phaeophyceae). Phycological Research 47, 217-223.

(4) Küpper H*, Küpper F, Spiller M (1998) In situ detection of heavy metal substituted chlorophylls in water plants. Photosynthesis Research 58, 123-133.

(3) Küpper H*, Küpper F, Spiller M (1997) Schwermetallchlorophylle in vivo. Junge Wissenschaft 45, 47-53.

(2) Küpper H*, Küpper F, Spiller M (1996) Environmental relevance of heavy metal substituted chlorophylls using the example of submersed water plants.- Journal of Experimental Botany 47, 259-266.

(1) Küpper F*, Küpper H, Spiller M (1996) Eine aggressive Wasserpflanze aus Australien und Neuseeland: Crassula helmsii (Kirk) Cockayne.- Floristische Rundbriefe 30, 24-29.

Reviews and Books; *=corresponding author

(11) Andresen E, Peiter E, Küpper H* (2018) Trace metal metabolism in plants. Journal of Experimental Botany 69, 909-954

(10) Küpper H* (2017) Lead Toxicity in Plants. in "Lead: Its Effects on Environment and Health", Vol. 17 of Metal Ions in Life Sciences,  Eds A. Sigel, H. Sigel, R.K.O. Sigel, Walter de Gruyter, GmbH, Berlin, Germany, 2017, pp. 491-500. DOI: 10.1515/9783110434330-015

(9) Küpper H*, Andresen E (2016) Mechanisms of metal toxicity in plants. Metallomics 8, 269-285.

(8) Leitenmaier B, Küpper H* (2013) Compartmentation and complexation of metals in hyperaccumulator plants. Frontiers in Plant Science, doi: 10.3389/fpls.2013.00374

(7) Küpper H*, Leitenmaier B (2013) Cadmium-Accumulating Plants. In: Cadmium: From Toxicity to Essentiality, Chapter 12, Volume 11 of series "Metal Ions in Life Sciences". (Eds: Sigel A, Sigel H, Sigel RKO). Springer Science + Business Media B.V., Dordrecht; pp. 373-394.

(6) Andresen E, Küpper H* (2013) Cadmium Toxicity in Plants. In: Cadmium: From Toxicity to Essentiality, Chapter 13, Volume 11 of series "Metal Ions in Life Sciences". (Eds: Sigel A, Sigel H, Sigel RKO). Springer Science + Business Media B.V., Dordrecht; pp. 395-414.

(5) Küpper H* (2011) Foreword of: Detoxification of Heavy metals (Eds: Sherameti I, Varma A) Springer, pp. v-xiv.

(4) Küpper H*, Kroneck PMH (2007) Nickel in the Environment and Its Role in the Metabolism of Plants and Cyanobacteria. In: Metal Ions in Life Sciences, Volume 2, chapter 2. (Eds: Sigel A, Sigel H, Sigel RKO). Wiley, Chichester; pp. 31-62.

(3) Küpper H*, Küpper FC, Spiller M (2006) [Heavy metal]-chlorophylls formed in vivo during heavy metal stress and degradation products formed during digestion, extraction and storage of plant material. In: Chlorophylls and Bacteriochlorophylls: Biochemistry, Biophysics, Functions and Applications (B. Grimm, R. Porra, W. Rüdiger and H. Scheer, eds.), Vol. 25 of series ”Advances in Photosynthesis and Respiration” (Series editor: Govindjee), chapter 5. Kluwer Academic Publishers, Dordrecht; pp. 67-77.

(2) Küpper H*, Kroneck PMH (2005) Heavy metal uptake by plants and cyanobacteria. In: Metal Ions in Biological Systems, Band 44, Kapitel 5. (Eds: Sigel A, Sigel H, Sigel RKO). Marcel Dekker, Inc., New York; pp. 97-142

(1) Küpper, H* (2001) Physiology of hyperaccumulator and non-accumulator plants: heavy metal uptake, transport, compartmentation, stress and resistance. (Doctoral thesis). UFO Atelier für Gestaltung und Verlag, Allensbach (ISBN 3-935511-07-8)

designed by Hendrik Küpper, last modified 2 April 2021